Analisis Pertumbuhan dan Kandungan Total Flavonoid Kultur Kalus Krisan (Chrysanthemum morifolium Ramat) dengan Pemberian Asam 2,4-diklorofenoksiasetat (2,4-d) dan Air Kelapa
DOI:
https://doi.org/10.33541/pro-life.v8i1.2781Keywords:
Zanthoxylum acanthopodium, essential oil, anti-microbialAbstract
Chrysanthemum morifolium contains flavonoids which have antioxidant properties and was useful in treating various diseases. The production of flavonoids from plants can be done in vitro through callus culture. Auxin 2,4-D growth regulators and organic compounds such as coconut water added to the media can affect the success of callus formation and secondary metabolite synthesis. The purpose of this study was to obtain the best treatment of 2,4-D concentration and coconut water that can increase the growth and total flavonoid levels of C. morifolium callus cultures. The method used in this research was the experimental method completely randomized design (CRD) with 8 treatments, namely 1 ppm 2,4-D + 10% coconut water (CW); 2 ppm 2,4-D + 10% CW; 3 ppm 2,4-D + 10% CW ; 4 ppm 2,4-D + 10% CW; 1 ppm 2,4-D; 2 ppm 2,4-D; 3 ppm 2,4-D; 4 ppm 2,4-D in 4 replication. The results showed that the treatment of 4 ppm 2,4-D and 10% CW was the best treatment combination, resulting in an average callus size (1.4 cm), fresh weight (0.19 grams), dry weight (0.16 gram) and total flavonoid content (1.873 mgQE/g)
References
Ariati SN, W Waeniati, Muslimin, & IN Suwastika. 2012. Induksi tanaman kakao pada medium MS dengan penambahan 2,4-D. Jurnal Natural Science, 1(1): 74-84.
Chang CC, MH Yang, HM Wen, & JC Chern. 2002. Estimation of total flavanoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal, 10 (3): 178-182.
Dwi NM, Waenati, Muslimin, & IN Suwastika. 2012. Pengaruh penambahan air kelapa dan berbagai konsentrasi hormon 2,4-D pada medium MS dalam menginduksi kalus tanaman anggur hijau (Vitis vinifera L.). Jurnal Natural Science. 1(1): 53-62.
Gunawan LW. 1992. Teknik Kultur Jaringan. Laboratorium Kultur Jaringan Tanaman PAU Bioteknologi IPB, Bogor.
Hidayat H. 2007. Induksi pertumbuhan eksplan endosperm ulin dengan IAA dan Kinetin. Agritop, 26(4):147-152.
Hoesen DSH, Witjaksono dan LA Sukamto. 2008. Induksi kalus dan organogenesis kultur in vitro Dendrobium lineale Rofle. Berita Biologi, 9(3): 333-341.
Huan LVT, T Takamura, & M Tanaka. 2004. Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid. Plant Science, 166(6): 1443-1449.
Indah PN & D Ermavitalini. 2013. Induksi Kalus Daun Nyamplung (Calophyllum inophylum Linn.) pada Beberapa Kombinasi Konsentrasi 6-
Benzylaminopurine (BAP) dan 2,4- Dichlorophenoxyacetic acid (2,4-D). Jurnal Sains dan Seni POMITS, 2(1): 2337-352
Isah T, S Umar, M Mujib, MP Sharma, PE Rajasekharan, N Zafar, & A Frukh. 2018. Secondary metabolism of pharmaceuticals in the plant in vitro cultures: strategies, approaches andlimitations to achieving higher yield. J. Plant Cell Tiss Organ Cult, 132: 239- 265.
Iwase A, M Ohme-Takagi, & K Sugimoto. 2011. WIND1: A key molecular switch for plant cell dedifferentiation plant signal. Behav. 6 (12): 1943-1945.
Jain SM. 2012. Date palm biotechnology: current status and prospective-an overview. Emir. J. Food Agric, 24 (5): 386-399.
Jan T, B Naqvi, R Qadri, & M Nisar. M. 2015. Effect of age of cultures and hormones on the synthesis of secondary metabolites from callus of Salvia santolinifolia (Boiss), a medicinal herb. European Journal of Biotechnology and Bioscience, 3(11): 19-22.
Jung EK. 2009. Chemical composition and antimicrobial activity of the essential oil of Chrysanthemum indicum against oral bacteria. J. Bacteriol, 19(2): 61-69.
Kalia R, JK Katnoria, & AK Nagpal. 2016. Antitumor activity of aqueous leaf extract of different cultivars of Chrysanthemum R. using potato disc tumor assay. Journal Pharmacuetical Sciences and Research, 8(11): 1262-1265.
Karimi N, HR Ghasmpour, & M Yari 2014. Effect of different growth regulators on callus induction and plant regeneration of satureja species. Annual Research & Review in Biology, 4(16): 2646-2654.
Kristina NN & SF Syahid. 2012. Pengaruh air kelapa terhadap muliplikasi tunas in vitro, produksi rimpang, dan kandungan xanthorizol temulawak di lapangan. Jurnal Littri, 18(3): 125-134.
Latifah R, T Suhermiatin, & N Ermawati. 2017. Optimasi pertumbuhan plantlet Cattleya melalui kombinasi kekuatan media Murashige-Skoog dan bahan organik. Jurnal Agriprima, 1(1): 59-68.
Lestari EG. 2011. Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan. Jurnal AgroBiogen, 7(1): 63-68.
Leupin E. 2000. Somatic embryogenesis from leaf callus devired from maturetress of the cycad Ceratozamia hildae (Gymnospermae). Journal Plant Cell, Tissue and Organ Culture, 40: 25- 31.
Lu N, EL Bernardo, C Tippayadarapani, M Takagaki, N Kagawa, & W Yamori,
W. 2017. Growth and accumulation of secondary metabolites in perilla as affected by photosynthetic photon flux density and electrical conductivity of the nutrient solution. Frontiers in Plant Science Plant Breeding, 8: 1-12.
Miyanaga K, M Seki, & S Furusaki. 2000. Analysis of pigmentation in individual cultured plant cells using an image processing system. Biotechnology Letters, 22: 977-981.
Mufarrikha L, N Herlina, & E Widaryanto 2014. Respon dua kultivar tanaman krisan (Chrysanthemum morifolium) pada berbagai lama penambahan cahaya buatan. Jurnal Produksi Tanaman, 2(1): 10-16.
Orihara Y, JW Yang, N Komiya, K Koge, & T Yoshikawa. 2002. Abietane diterpenoid from suspension cultured cells of Torreya nucifera var. radicans. Phytochemistry, 59(4): 385-389.
Pan Y, L Lin, S Xiao, Z Chen, S Sarsaiya, S Zhang, YS Guan, H Liu, & D Xu. 2020. Callus growth kinetics and accumulation of secondary metabolites of Bletilla striata Rchb.f. using a callus suspension culture. PLoS ONE, 15(2).
Prakash NKU, S Bhuvaneswari, N Sripriya, R Arulmozhi, K Kavitha, R Aravitha, & B Bharathiraja. 2014. Studie on phytochemistry, antioxidant, antibacterial, larvicidal, and pesticidal activities of aromatic plants from Yelagiri hills. International Journal of Pharmacy and Pharmacetical Sciences, 6(5): 1-4.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Tia Setiawati, Anggita Levi Astuti, Mohamad Nurzaman, Nining Ratningsih
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
.svg_.png){kind=logo}
