Kandungan Fitokimia dan Uji Toksisitas Jamu Susut Perut

Albert Jackson Yang; Fri Rahmawati; John Jackson Yang

doi:10.33541/pro-life.v12i3.7079

Authors

Albert Jackson Yang Departemen Fisiologi Fakultas Kedokteran Universitas Kristen Indonesia, Jakarta
Fri Rahmawati Departemen Biokimia Kedokteran Fakultas Kedokteran Universitas Kristen Indonesia, Jakarta
John Jackson Yang Departemen Biokimia Kedokteran Fakultas Kedokteran Universitas Kristen Indonesia, Jakarta

DOI:

https://doi.org/10.33541/pro-life.v12i3.7079

Keywords:

BLST, phytochemicals, jamu susut perut , toxicity

Abstract

Jamu Susut Perut is a traditional Indonesian herbal preparation widely used as a complementary therapy to support weight loss and reduce abdominal fat. It commonly contains medicinal plants such as Guazuma ulmifolia (Jati belanda), Curcuma longa (Turmeric), Zingiber purpureum (Bangle), Murraya paniculata (Kemuning), Orthosiphon aristatus (Cat’s whiskers), Gallae (Majakanis), Areca catechu (Betel nut), and Piperis folium (Betel leaf). These plants are rich in bioactive compounds including flavonoids, polyphenols, alkaloids, and saponins, which possess lipolytic, anti-inflammatory, and hepatoprotective properties that may enhance fat metabolism and lower blood lipid levels. This study aims to identify the phytochemical compounds in Jamu Susut Perut (X) using the Harborne method and to determine its toxicity against shrimp larvae (Artemia salina Leach) through the Brine Shrimp Lethality Test (BSLT). The LC₅₀ (lethal concentration 50%) value was calculated using probit analysis in SPSS and Microsoft Excel. The phytochemical screening revealed the presence of triterpenoids, quinones, flavonoids, and saponins in the herbal formulation. The toxicity test showed that Jamu Susut Perut (X) had an LC₅₀ value of 547.92 ppm, indicating toxicity to shrimp larvae since the value is below 1000 ppm. In conclusion, Jamu Susut Perut (X) contains bioactive compounds with potential pharmacological effects but also exhibits toxic properties at certain concentrations. This research provides scientific data to ensure the safe use of Jamu Susut Perut as a traditional herbal medicine for consumers.

References

Ahmad, R., Hussain, A. I., Ahmad, S., Hameed, S., & Shariati, M. A. (2021). Phytochemical composition and biological activities of Zingiber officinale: A review. Plants, 10(8), 1689.

Anita, M., Darmawan, E., & Wahyuni, D. (2021). Potential of Guazuma ulmifolia and Curcuma longa in lowering lipid profiles in rat models of hyperlipidemia. Jurnal Fitofarmaka Indonesia, 8(2), 95–102.

Badan Pengawas Obat dan Makanan Republik Indonesia. (2023). Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia Nomor 25 Tahun 2023 tentang Kriteria dan Tata Laksana Registrasi Obat Alam. Biro Hukum Badan Pengawas Obat dan Makanan Republik Indonesia.

Bratsch, T. (2007). Understanding oak galls. University of Illinois Extension. https://web.extension.illinois.edu/ccgms/downloads/42438

Chen, Y., Wang, X., Li, H., & Zhao, J. (2025). Phytochemicals from Moringa oleifera as potential inhibitors of BCL-2: Insights from molecular docking. Frontiers in Pharmacology, 16, 1542098.

Dalimartha, S. (2009). Atlas tumbuhan obat Indonesia (Jilid 6, pp. 153–154). Jakarta: Pustaka Bunda.

Dewoto, H. R. (2007). Pengembangan obat tradisional Indonesia menjadi fitofarmaka. Majalah Kedokteran Indonesia, 57(7), 205–211.

Docimo, T., Celano, R., Lambiase, A., Di Sanzo, R., Serio, S., Santoro, V., Coccetti, P., Russo, M., Rastrelli, L., & Piccinelli, A. L. (2024). Exploring influence of production area and harvest time on specialized metabolite content of Glycyrrhiza glabra leaves and evaluation of antioxidant and anti-aging properties. Antioxidants, 13(1), 93.

Galo, A. (1999). Studi efek jamu susut perut terhadap kadar kolesterol total darah kelinci jantan [Disertasi, Universitas Hasanuddin].

Han, N., & Bakovic, M. (2015). Biologically active triterpenoids and their cardioprotective and anti-inflammatory effects. Journal of Bioanalysis & Biomedicine, 12(5), 1948–1959.

Harborne, J. B., Williams, C. A., & Zhang, H. (2021). Galangin: A natural flavonoid with anticancer potential. Phytochemistry Reviews, 20(6), 1259–1275.

Jelita, S. F., Setyowati, G. W., & Ferdinand, M. (2020). Uji toksisitas infusa Acalypha siamensis dengan metode brine shrimp lethality test (BSLT). Farmaka, 18(1), 14–22.

Katno, P. S., Prapti, I., Rahmawati, N., & Mujahid, R. (2008). Tingkat manfaat, keamanan dan efektivitas tanaman obat dan obat tradisional. Balai Penelitian Tanaman Obat Tawangmangu.

Menteri Kesehatan Republik Indonesia. (2012). Peraturan Menteri Kesehatan Republik Indonesia Nomor 007 Tahun 2012 tentang Registrasi Obat Tradisional.

Meng, Q., Guo, J., Sun, W., & Wang, X. (2022). Flavonoids and bone health: A review of molecular mechanisms. Molecular Medicine Reports, 25(6), 169.

Meyer, B., Ferrigni, N., Putnam, J., Jacobsen, L., Nichols, D., & McLaughlin, J. L. (1982). Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica, 45(5), 31–34.

Nugroho, A. P. (2022). Kebijakan afirmatif untuk obat tradisional di Indonesia. Kajian, 27(1), 57–70.

Oktora, L., & Kumala, R. (2006). Pemanfaatan obat tradisional dengan pertimbangan manfaat dan keamanannya. Majalah Ilmu Kefarmasian, 3(1), 1–7.

Pamadyo, S., Novianto, F., & Mujahid, R. (2015). Observasi klinik ramuan jamu untuk menurunkan berat badan. Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice), 2(1).

Pratikno, H. (2010). Pengaruh ekstrak kunyit (Curcuma domestica Vahl) terhadap bobot badan ayam broiler (Gallus sp.). Anatomi Fisiologi, 18(2), 39–46.

Puspitasari, M. D., Wulandari, R., & Santosa, H. (2019). Pengaruh pemberian ekstrak bangle dan kemuning terhadap ekspresi enzim lipase pankreas pada model tikus obesitas. Jurnal Biomedik Veteriner, 13(2), 66–72.

Qaderi, M. M., Martel, A. B., & Strugnell, C. A. (2023). Environmental factors regulate plant secondary metabolites. Plants, 12(3), 447.

Rahim, A. J. B. N., & Iyabu, H. (2014). Analisis fitokimia tumbuhan obat di Kabupaten Boalemo. Fakultas Matematika dan IPA, Universitas Negeri Gorontalo, 2(1).

Rahmasiah, R., Hadiq, S., Nurpati, A., & Hasma, H. (2024). Sosialisasi penggunaan obat tradisional di Desa Tuncung Kecamatan Maiwa Kabupaten Enrekang. Jurnal Inovasi dan Pengabdian Masyarakat (JIPengMas), 4(2), 31–36.

Ramadhani, F., Nurmala, I., & Syafruddin, D. (2022). Efek kombinasi ekstrak daun jati belanda dan kunyit terhadap penurunan berat badan dan profil lipid tikus obesitas. Jurnal Ilmu Kefarmasian Indonesia, 20(1), 45–52.

Redha, A. (2010). Flavonoids: Structure, antioxidative properties and their role in biological systems. Belian’s Journal, 2(9).

Safitri, A. (2016). Tanaman ajaib! Basmi penyakit dengan TOGA (tanaman obat keluarga). Depok: Bibit Publisher.

Salm, S., Rutz, J., van den Akker, M., Blaheta, R. A., & Bachmeier, B. E. (2023). Current state of research on the clinical benefits of herbal medicines for non-life-threatening ailments. Frontiers in Pharmacology, 14, 1234701.

Sangi, M., Runtuwene, M., Simbala, H., & Makang, V. (2008). Analisis fitokimia tumbuhan obat di Kabupaten Minahasa Utara. Chemistry Progress, 1(1), 47–53.

Suharmiati, H. L. (2006). Cara benar meracik obat tradisional. Jakarta: Agromedia Pustaka.

Sun, L., Zhang, L., Zhang, Y., Zheng, Y., Du, Y., & Li, J. (2018). Dietary vitamin C intake and the risk of osteoporosis: A meta-analysis. Osteoporosis International, 29(3), 491–501.

Syahrir, N. H. A., Afendi, F. M., & Susetyo, B. (2016). Efek sinergis bahan aktif tanaman obat berbasiskan jejaring dengan protein target. Jurnal Jamu Indonesia, 1(1), 35–46.

Utami, S. M., Febriyanti, E., Karimatulhajj, H., Issusilaningtyas, E., Rosita, M. E., Ariawan, M. W., ... & Pertiwi, Y. (2024). Pengembangan obat tradisional.

Wali, M., Haneda, N. F., & Maryana, N. (2014). Identifikasi kandungan kimia bermanfaat pada daun jabon merah dan putih (Anthocephalus spp.). Jurnal Silvikultur Tropika, 5(2), 77–83.

Yuliani, S. H., Sari, R., & Muchtaridi, M. (2020). Antiobesity potential of Indonesian herbal formulations: A systematic review. Journal of Advanced Pharmaceutical Technology & Research, 11(3), 109–116.

Zein, U. (2005). Pemanfaatan tumbuhan obat dalam upaya pemeliharaan kesehatan.

Zhang, R., Zhu, X., Bai, H., & Ning, K. (2019). Network pharmacology databases for traditional Chinese medicine: Review and assessment. Frontiers in Pharmacology, 10, 123.

Zhou, D., Zhang, H., Xue, X., Tao, Y., Wang, S., Ren, X., & Su, J. (2022). Safety evaluation of natural drugs in chronic skeletal disorders: A literature review of clinical trials in the past 20 years. Frontiers in Pharmacology, 12, 801287.

Zhou, Y., Li, Y., Zhang, J., & Chen, Y. (2022). Effects of flavonoid intake on bone mineral density and osteoporosis risk: Evidence from NHANES 2011–2018. BMC Public Health, 22(1), 1753.