PENGARUH FRAGMENTASI DNA SPERMA TERHADAP ANGKA FERTILISASI DAN EMBRIOGENESIS
Abstrak
Latar Belakang: Pemeriksaan fragmentasi DNA sperma sudah mulai banyak digunakan dalam upaya memperoleh keturunan pada pasangan subfertil yang mengikuti program bayi tabung. Pemeriksaan sperm chromatin dispersion merupakan salah satu pemeriksaan dengan metodologi yang simpel dan relatif mudah dilakukan untuk kepentingan klinis.
Tujuan: untuk mengetahui pengaruh fragmentasi DNA sperma terhadap angka fertilisasi dan embriogenesis.
Metode: Penelitian observasional ini dilakukan dengan mengambil sampel semen pria dari pasangan infertil yang mengikuti program IVF-ICSI, dilakukan pemeriksaan analisis sperma WHO dan penilaian indeks fragmentasi DNA sperma. Setelah dilakukan ICSI kemudian dilakukan penilaian fertilisasi dan embriogenesis. Selanjutnya dilakukan analisis dengan menghitung korelasi. Rancangan penelitian adalah cross sectional untuk melihat korelasi antara indeks fragmentasi DNA sperma dengan angka fertilisasi dan embriogenesis. Untuk analisis beda rerata 2 kategori menggunakan independent sample t-test sedangkan yang lebih dari 2 kategori memakai anova. Analisis untuk jenis variabel kategorikal menggunakan chi-square test.
Hasil: Pada periode penelitian selama 10 bulan, jumlah subyek penelitian yang sesuai dengan kriteria inklusi adalah 29 pasangan infertil dengan 29 sampel sperma ejakulat dan 200 oosit. Dari analisis indeks fragmentasi DNA sperma dengan angka fertilisasi terlihat bahwa fertilisasi yang baik (Z1 dan Z2) lebih banyak terdapat pada kelompok dengan indeks fragmentasi DNA sperma £30%, dan berbeda bermakna secara statistik dengan p<0.05, dan dari analisis indeks fragmentasi DNA sperma dengan skoring embrio terlihat skoring embrio yang lebih baik (grade A dan B) pada kelompok indeks fragmentasi DNA sperma £30%, yang tidak bermakna secara statistik.
Kesimpulan: Angka fragmentasi DNA sperma dapat menunjukkan hubungan negatif dengan angka fertilisasi tetapi tidak dapat menunjukkan hubungan yang bermakna secara statistik dengan angka embriogenesis. Terlihat hubungan yang bermakna secara statistik antara parameter analisis sperma WHO dengan indeks fragmentasi DNA yang diperiksa menggunakan metode SCD.
Referensi
Agarwal, A., & Allamaneni, S. S. R. (2004). The effect of sperm DNA damage on assisted reproduction outcomes. A review. Minerva Ginecologica, 56(3), 235–245. https://europepmc.org/article/med/15258535
Benchaib, M., Braun, V., Lornage, J., Hadj, S., Salle, B., Lejeune, H., & Guérin, J. F. (2003). Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Human Reproduction, 18(5), 1023–1028. https://doi.org/10.1093/HUMREP/DEG228
Brazil, C. (2010). Practical semen analysis: from A to Z. Asian Journal of Andrology, 12(1), 14. https://doi.org/10.1038/AJA.2008.51
Cohen-Bacrie, P., Belloc, S., Ménézo, Y. J. R., Clement, P., Hamidi, J., & Benkhalifa, M. (2009). Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients. Fertility and Sterility, 91(5), 1801–1805. https://doi.org/10.1016/J.FERTNSTERT.2008.01.086
Dohle, G. R., Zsolt, K., Jungwirth, A., Diemer, T., Giwercman, A., & Krausz, C. (2002). GUIDELINES FOR THE INVESTIGATION AND TREATMENT OF MALE INFERTILITY. In Eur Urol (Vol. 42, Issue 4).
Evenson, D. P., & Wixon, R. (2006). Clinical aspects of sperm DNA fragmentation detection and male infertility. Theriogenology, 65(5), 979–991. https://doi.org/10.1016/J.THERIOGENOLOGY.2005.09.011
Fernández, J. L., Muriel, L., Rivero, M. T., Goyanes, V., Vazquez, R., & Alvarez, J. G. (2003). The Sperm Chromatin Dispersion Test: A Simple Method for the Determination of Sperm DNA Fragmentation. Journal of Andrology, 24(1), 59–66. https://doi.org/10.1002/J.1939-4640.2003.TB02641.X
Gil-Villa, A. M., Cardona-Maya, W., Agarwal, A., Sharma, R., & Cadavid, Á. (2009). Role of male factor in early recurrent embryo loss: do antioxidants have any effect? Fertility and Sterility, 92(2), 565–571. https://doi.org/10.1016/J.FERTNSTERT.2008.07.1715
Hourcade, J. D., Pérez-Crespo, M., Fernández-González, R., Pintado, B., & Gutiérrez-Adán, A. (2010). Selection against spermatozoa with fragmented DNA after postovulatory mating depends on the type of damage. Reproductive Biology and Endocrinology, 8(1), 1–11. https://doi.org/10.1186/1477-7827-8-9/TABLES/4
Khalili, M. A., Aghaie-Maybodi, F., Anvari, M., & Talebi, A. R. (2006). SPERM NUCLEAR DNA IN EJACULATES OF FERTILE AND INFERTILE MEN CORRELATION WITH SEMEN PARAMETERS. In Urology Journal (Vol. 3, Issue 3, pp. 154–159). UROLOGY JOURNAL. https://sid.ir/paper/270712/en
Knez, K., Zorn, B., Tomazevic, T., Vrtacnik-Bokal, E., & Virant-Klun, I. (2011). The IMSI procedure improves poor embryo development in the same infertile couples with poor semen quality: A comparative prospective randomized study. Reproductive Biology and Endocrinology, 9(1), 1–8. https://doi.org/10.1186/1477-7827-9-123/TABLES/2
Larson, K. L., DeJonge, C. J., Barnes, A. M., Jost, L. K., & Evenson, D. P. (2000). Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Human Reproduction, 15(8), 1717–1722. https://doi.org/10.1093/HUMREP/15.8.1717
Lin, M. H., Kuo-Kuang Lee, R., Li, S. H., Lu, C. H., Sun, F. J., & Hwu, Y. M. (2008). Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates. Fertility and Sterility, 90(2), 352–359. https://doi.org/10.1016/J.FERTNSTERT.2007.06.018
Manicardi, G. C., Bianchi, P. G., Pantano, S., Azzoni, P., Bizzaro, D., Bianchi, U., & Sakkas, D. (1995). Presence of Endogenous Nicks in DNA of Ejaculated Human Spermatozoa and its Relationship to Chromomycin A3 Accessibility. Biology of Reproduction, 52(4), 864–867. https://doi.org/10.1095/BIOLREPROD52.4.864
Moustafa, M., Sharma, R. K., Thornton, J., Mascha, E., Abdel-Hafez, M. A., Thomas, A. J., & Agarwal, A. (2004). Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Human Reproduction, 19(1), 129–138. https://doi.org/10.1093/HUMREP/DEH024
Scott, L., Alvero, R., Leondires, M., & Miller, B. (2000). The morphology of human pronuclear embryos is positively related to blastocyst development and implantation. Human Reproduction, 15(11), 2394–2403. https://doi.org/10.1093/HUMREP/15.11.2394
Shaman, J. A., Yamauchi, Y., & Ward, W. S. (2007). Sperm DNA fragmentation: awakening the sleeping genome. Biochemical Society Transactions, 35(3), 626–628. https://doi.org/10.1042/BST0350626
Sheikh, N., Amiri, I., Farimani, M., Najafi, R., & Hadeie, J. (2008). CORRELATION BETWEEN SPERM PARAMETERS AND SPERM DNA FRAGMENTATION IN FERTILE AND INFERTILE MEN. In Iranian Journal of Reproductive Medicine (Vol. 6, Issue 1, pp. 13–18). INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE). https://sid.ir/paper/295142/en
Simon, L., Lutton, D., McManus, J., & Lewis, S. E. M. (2011). Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success. Fertility and Sterility, 95(2), 652–657. https://doi.org/10.1016/J.FERTNSTERT.2010.08.019
Tamburrino, L., Marchiani, S., Montoya, M., Elia Marino, F., Natali, I., Cambi, M., Forti, G., Baldi, E., & Muratori, M. (2012). Mechanisms and clinical correlates of sperm DNA damage. Asian Journal of Andrology, 14(1), 24. https://doi.org/10.1038/AJA.2011.59
Taylor, H. S., Preceded by (work): Fritz, M. A., Pal, L., & Seli, E. (n.d.). Speroff’s clinical gynecologic endocrinology and infertility. 1318. Retrieved July 19, 2024, from https://www.wolterskluwer.com/en/solutions/ovid/speroffs-clinical-gynecologic-endocrinology-and-infertility-711
Tesarik, J., Greco, E., & Mendoza, C. (2004). Late, but not early, paternal effect on human embryo development is related to sperm DNA fragmentation. Human Reproduction, 19(3), 611–615. https://doi.org/10.1093/HUMREP/DEH127
Zini, A., Bielecki, R., Phang, D., & Zenzes, M. T. (2001). Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertility and Sterility, 75(4), 674–677. https://doi.org/10.1016/S0015-0282(00)01796-9
- View 0 times Download 0 times Pdf (English)
##submission.copyrightStatement##
##submission.license.cc.by4.footer##
