Aplikasi Asam Humat dan Pupuk Organik Cair Ekstrak Rumput Laut untuk Meningkatkan Pertumbuhan dan Kandungan Flavonoid Brassica juncea L.
Abstract
This research was conducted with the aim of obtaining the optimal dose of humic acid (HA) and liquid organic fertilizer (LOF) from seaweed extract that can enhance the growth and total flavonoid content of Brassica juncea L. The method used in this study was experimental with a randomized complete block design with two factors. The first factor was HA, consisting of 4 dose levels: 0, 4, 8, and 12 g/kg, while the second factor was LOF from seaweed extract, consisting of 4 dose levels: 0, 1, 2, and 3 mL/L. Each treatment was replicated four times. Parameters were observed on the 42nd day, including plant height, leaf number, leaf area, dry weight, and total flavonoids. Data were analyzed using Analysis of Variance (ANOVA) and followed by Duncan's Multiple Range Test with a confidence level of 95%. The results showed that the dose of 4 g/kg HA and 2 mL/L LOF was the best dose for enhancing growth and total flavonoid content, producing an average plant height of 19.25 cm, a leaf number of 5.75, a total leaf area of 179.8 cm2, a dry weight of 0.57 grams, and a total flavonoid content of 0.106 mg QE/g sample.
References
Adianti, R., Proklamasiningsih, E., & Sasongko, N. D. (2019). Pertumbuhan dan kandungan flavonoid bayam merah (Alternanthera amoena Voss) pada media tanam dengan pemberian asam humat dan urea. BioEksakta : Jurnal Ilmiah Biologi Unsoed, 1(2), 91–95.
Aina, O. E., Amoo, S. O., Mugivhisa, L. L., & Olowoyo, J. O. (2019). Effect of organic and inorganic sources of nutrients on the bioacttve compounds and antioxidant activity of tomato. Applied Ecology and Environmental Research, 17(2), 3681–3694. https://doi.org/10.15666/aeer/1702_36813694
Al-Taey, D. K. A., Al-Shareefi, M. J. H., Mijwel, A. K., Al-Tawaha, A. R., & Al-Tawaha, A. R. (2019). The beneficial effects of bio-fertilizers combinations and humic acid on growth, yield parameters and nitrogen content of broccoli grown under drip irrigation system. Bulgarian Journal of Agricultural Science, 25(5), 959–966.
Alkharpotly, A., Mohamed, R., Shehata, M., & Awad, A. (2017). Impact of Soil Humic Acid Soil Application and Seaweed Extract Foliar Spray on Growth, Yield, and Fruits Quality of Strawberry Plants Grown under Aswan Conditions. Journal of Soil Sciences and Agricultural Engineering, 8(6), 307–315. https://doi.org/10.21608/jssae.2017.37496
Chang, C., Yang, M., Wen, H., & Chern, J. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 178–182.
Diriba-Shiferaw, G., Nigussie-Dechassa, R., Kebede, W., J., J. S., & Getachew, T. (2013). Bulb quality of Garlic (Allium sativum L.) as influenced by the application of inorganic fertilizers. African Journal of Agricultural Research, 9(8), 784–796. https://doi.org/10.5897/ajar2013.7723
Eskandari, H., & Kazemi, K. (2012). Changes in Germination Properties of Rape (Brassica napus L.) as Affected by Hydropriming of Seeds. J. Basic. Appl. Sci. Res, 2(4), 3285–3288.
Fauziah, I., Proklamasiningsih, E., & Budisantoso, I. (2019). PengaruhAsam Humat pada Media Tanam Zeolit terhadap Pertumbuhan dan KandunganVitamin C Sawi Hijau (Brassica juncea). Jurnal Ilmiah Biologi Unsoed, 1(2), 17–21.
Gholami, H., Saharkhiz, M. J., Raouf Fard, F., Ghani, A., & Nadaf, F. (2018). Humic acid and vermicompost increased bioactive components, antioxidant activity and herb yield of Chicory (Cichorium intybus L.). Biocatalysis and Agricultural Biotechnology, 14, 286–292. https://doi.org/https://doi.org/10.1016/j.bcab.2018.03.021
Gomaa, A. M., & Ibrahim, H. (2020). Williams Banana Growth, Nutritional Status, Yield and Fruit Quality as Influenced by Spraying Humic Acid and Seaweed Extract. Journal of Plant Production, 11(11), 1121–1128. https://doi.org/10.21608/jpp.2020.130950
Haryanti, S. (2008). Respon Pertumbuhan Jumlah dan Luas Daun Nilam (Pogostemon cablin Benth) pada Tingkat Naungan yang Berbeda. Buletin Anatomi Fisiologi, XVI(2), 20–26.
Hassoon, A. S., Ussain, M. H., & Harby, H. H. (2018). Effect of spraying of humic acid on sepals extract content from some antioxidants for three varieties of rosella (Hibiscus sabdariffa L.). Plant Archives, 18(1), 1129–1133.
Heimler, D., Romani, A., & Ieri, F. (2017). Plant polyphenol content, soil fertilization and agricultural management: a review. European Food Research and Technology, 243(7), 1107–1115. https://doi.org/10.1007/s00217-016-2826-6
Hilmi, A., Laili, S., & Rahayu, T. (2018). Pengaruh Pemberian Limbah Biogas Cair dan Padat (Bio Slurry) sebagai Pupuk Organik terhadap Pertumbuhan Tanaman Sawi Hijau (Brassica juncea). Jurnal Sains ALlami, 1(1), 65–71. https://doi.org/10.33474/j.sa.v1i1.1417
Inri, I., Paling, S., & Alua, I. (2019). Lama Perendaman Benih Sawi Hijau (Brassica rapa var. parachinensis) Dalam Larutan Mikroorganisme Lokal (MoL) Bonggol Pisang Kepok (Musa paradisiaca L.) Terhadap Viabilitas Benih. In STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa (Vol. 12, Issue 01, pp. 11–20). https://doi.org/10.36456/stigma.vol12.no01.a1855
Istiqomah, I., & Serdani, A. D. (2018). Pertumbuhan dan Hasil Tanaman Sawi (Brassica juncea L. Var. Tosaka) pada Pemupukan Organik, Anorganik, dan Kombinasinya. Agroradix: Jurnal Ilmu Pertanian, 1(2), 1–8.
Jan, J. A., Nabi, G., Khan, M., Ahmad, S., Shah, P. S., Hussain, S., & Sehrish. (2020). Foliar Application of Humic Acid Improves Growth and Yield of Chilli (Capsicum annum L.) Varieties. Pakistan Journal of Agricultural Research, 33(3), 461–472. https://doi.org/10.17582/journal.pjar/2020/33.3.461.472
Jasim, A. H., & Al-amiri, K. A. H. (2020). Effect of Soil Mulching, Soil Phosphorus Fertilizer and Humic Acid on Broad Bean Yield. Plant Archives, 20(2), 6481–6484.
Khoirunnisa, I., & Sumiwi, S. A. (2019). Peran Flavonoid Pada Berbagai Aktifitas Farmakologi. Farmaka, 17(2), 131–142.
Kumar, M., PK Singh, KG Yadav, Ashutosh Chaurasiya, & Yadav, A. (2017). Effect of nitrogen and sulphur on growth and yield of mustard (Brassica juncea). Indian Journal of Agronomy, 38(2), 445–448. https://doi.org/10.59797/ija.v38i2.3922
Mahmoud, S. H., Salama, D. M., El-Tanahy, A. M. M., & Abd El-Samad, E. H. (2019). Utilization of seaweed (Sargassum vulgare) extract to enhance growth, yield and nutritional quality of red radish plants. Annals of Agricultural Sciences, 64(2), 167–175. https://doi.org/https://doi.org/10.1016/j.aoas.2019.11.002
Mahmoud, S., Marzouk, N. M., EL-Tanahy, A., & Abou-Hussein, S. (2018). Humic acid as soil drench and Effective seaweed enhanced growth and productivity of sweet potato plants. Middle East Journal of Applied Sciences, 8(2018), 1355–1363.
Marpaung, A. E., Karo, B., & Tarigan, R. (2014). Pemanfaatan Pupuk Organik Cair dan Teknik Penanaman dalam Peningkatan Pertumbuhan dan Hasil Kentang. Jurnal Hortikultura, 24(1), 49–55. https://doi.org/10.21082/jhort.v24n1.2014.p49-55
Munthe, K., Pane, E., & Panggabean, E. L. (2018). Budidaya Tanaman Sawi ( Brassica juncea L. ) Pada Media Tanam Yang Berbeda Secara Vertikultur. Agrotekma: Jurnal Agroteknologi Dan Ilmu Pertanian, 2(2), 138. https://doi.org/10.31289/agr.v2i2.1632
Nurmayulis, U., Utama, P., & Jannah, R. (2014). Pertumbuhan dan Hasil Tanaman Selada (Lactuca Sativa) yang Diberi Bahan Organik Kotoran Ayam Ditambah Beberapa Bioaktivator. Agrologia, 3(1). https://doi.org/10.30598/a.v3i1.259
Nuryani, E., Haryono, G., & Historiawati. (2019). Pengaruh Dosis dan Saat Pemberian Pupuk P terhadap Hasil Tanaman Buncis (Phaseolus vulgaris L .) Tipe Tegak. Jurnal Imu Pertanian Tropika Dan Subtropika, 4(1), 14–17.
Paul, N., Giri, U., Datta, A., Dhar, D., & Saha, D. (2017). Comparative Study of Organic Matter Vis-a-Vis Humic Acid on Change in Nutrients Availability in Rice-Mustard Cropping Sequence. International Journal of Agriculture, Environment and Biotechnology, 10(3), 303. https://doi.org/10.5958/2230-732x.2017.00038.9
Pramushinta, I. A. K. (2019). Pengaruh Pemberian Pupuk Organik Cair dari Limbah Cair Tahu dan Serbuk Tulang Ikan Bandeng terhadap Kandungan Flavonoid Daun Bayam Merah (Althernantera Amoena Voss). Journal Pharmasci, 4(2), 97–100. https://doi.org/10.53342/pharmasci.v4i2.143
Prasetyo, J., & Lazuardi, I. B. (2017). Pemaparan Teknologi Sonic Bloom Dengan Pemanfaatan Jenis MusikTerhadap Pertumbuhan Vegetatif Tanaman Selada Krop (Lactuca Sativa L). Jurnal Keteknikan Pertanian Tropis Dan …, 5(2), 189–199.
Pratama, M. R., & Nihayati, E. (2021). Pengaruh Berbagai Dosis Pengapuran dan Pupuk Kandang Terhadap Pertumbuhan dan Kandungan Senyawa Antosianin pada Tanaman Coleus (Coleus scutellarioides L.) Effect of Liming and Manure Doses on the Growth and Content of Anthocyanin Compounds on Coleus (Cole. Journal of Agricultural Science, 2021(1), 11–20.
Pratiwi, A. (2017). Effect of nitrogen fertilizer to the flavonoid content of red amaranth (Amaranthus gangeticus L.). Pharmaciana, 7(1), 87. https://doi.org/10.12928/pharmaciana.v7i1.4213
Radite, S., & Simanjuntak, B. H. (2020). Penggunaan Asam Humat Sebagai Pelapis Urea Terhadap Pertumbuhan dan Hasil Tanaman pakcoy (Brassica rapa L.). Agriland, 8(1), 72–78.
Rahman, K. M. A., & Zhang, D. (2018). Effects of fertilizer broadcasting on the excessive use of inorganic fertilizers and environmental sustainability. Sustainability (Switzerland), 10(3). https://doi.org/10.3390/su10030759
Raksun, A., & Karnan, K. (2019). Pembinaan Masyarakat dalam Budidaya Tanaman Cabai Rawit dengan Sistem Bedengan Lahan dan Aplikasi Mulsa Plastik. Jurnal Pengabdian Magister Pendidikan IPA, 2(1), 1–7. https://doi.org/10.29303/jpmpi.v1i2.240
Rizal, S. (2017). pengaruh nutrisi terhadap perrumbuhan tanaman sawi pakcoy (Brasicca rapa L.) yang di tanam secara hidroponik. Sainmatika, 14(1), 38–44.
Sakr, M. T., Ibrahim, H. M., ElAwady, A. E., & AboELMakarem, A. A. (2019). Effect of humic acid, seaweed extract and essential oils as antioxidants on pre-and post-harvest quality of red radish plants. Horticulture International Journal, 3(3), 129–138. https://doi.org/10.15406/hij.2019.03.00120
Sandepogu, M., Shukla, P. S., Asiedu, S., Yurgel, S., & Prithiviraj, B. (2019). Combination of ascophyllum nodosum extract and humic acid improve early growth and reduces post-harvest loss of lettuce and Spinach. Agriculture (Switzerland), 9(11). https://doi.org/10.3390/agriculture9110240
Seong, G.-U., Hwang, I.-W., & Chung, S.-K. (2016). Antioxidant capacities and polyphenolics of Chinese cabbage (Brassica rapa L. ssp. Pekinensis) leaves. Food Chemistry, 199, 612–618. https://doi.org/https://doi.org/10.1016/j.foodchem.2015.12.066
Siregar, M. (2017). Respon pemberian nutrisi AB mix pada sistem tanam hidroponik terhadap pertumbuhan dan produksi tanaman sawi ( Brassica juncea ). Journal of Animal Science and Agronomy Panca Budi, 2(2), 18–24.
Supriyatin, E., & Pratiwi, A. (2019). Pengaruh pupuk organik cair limbah padat bakpia dan cair tempe terhadap pertumbuhan tanaman sawi hijau (Brassica rapa L.). Symposium of Biology Education (Symbion), 2, 38–47. https://doi.org/10.26555/symbion.3507
Wahyuningsih, W., Proklamasiningsih, E., & Dwiati, M. (2016). Serapan Fosfor dan Pertumbuhan Kedelai(Glycine max) pada Tanah Ultisol dengan Pemberian Asam Humat. Biosfera, 33(2), 66–70. https://doi.org/10.20884/1.mib.2016.33.2.345
Walanda, W. P., Suryanto, E., & Abidjulu, J. (2016). Pengaruh Ekstrak Kasar Yang Mengandung Enzim Peroksidasi Dari Sawi Hijau (Brassica juncea) Terhadap Aktivtas Antioksidan Ekstrak Tongkol Jagung (Zea mays L.). PHARMACONJurnal Ilmiah Farmasi-UNSRAT, 5(3).
Wenda, M., Hidayati, S., & Purwanti. (2017). Aplikasi pupuk organik cair dan komposisi media tanam terhadap hasil tanaman selada (Lactuca sativa L.). Gontor AGROTECH Science Journal, 3(2), 99–118. https://doi.org/10.21111/agrotech.
Wijaya, R. (2018). Pengaruh Konsentrasi GA3 dan Dosis Pupuk N Terhadap Pertumbuhan dan Hasil Tanaman Selada (Lactuca sativa L.). Median : Jurnal Ilmu Ilmu Eksakta, 10(1), 1–8. https://doi.org/10.33506/md.v10i1.366
Wijiyanti, P., Hastuti, E. D., & Haryanti, S. (2019). Pengaruh Masa Inkubasi Pupuk dari Air Cucian Beras Terhadap Pertumbuhan Tanaman Sawi Hijau (Brassica juncea L.). Buletin Anatomi Dan Fisiologi, 4(1), 21–28. https://doi.org/10.14710/baf.4.1.2019.21-28
Winkel-Shirley, B. (2002). Biosynthesis of flavonoids and effects of stress. Current Opinion in Plant Biology, 5(3), 218–223. https://doi.org/10.1016/s1369-5266(02)00256-x
Yusuf, R., Bahrudin, Mas’ud, H., Syakur, A., Afriana, D. S., Kalaba, Y., & Kristiansen, P. (2020). Application of local seaweed extracts on growth and yield of mustard greens (Brassica juncea L.). IOP Conference Series: Earth and Environmental Science, 484(1). https://doi.org/10.1088/1755-1315/484/1/012066
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