Uji Aktivitas Antibakteri Ekstrak Kulit Buah Kawista (Limonia acidissima L.) Asal Desa Pasinan, Kabupaten Pasuruan terhadap Koloni Salmonella sp. secara In Vitro

Sisca Desi Prastyaningtias; Sari Dian A.S

doi:10.33541/jpvol6Iss2pp102

Sisca Desi Prastyaningtias Pendidikan Biologi, Fakultas Ilmu Pendidikan, Institut Teknologi dan Sains Nahdlatul Ulama Pasuruan
Sari Dian A.S Pendidikan Fisika, Fakultas Ilmu Pendidikan, Institut Teknologi dan Sains Nahdlatul Ulama Pasuruan

DOI: https://doi.org/10.33541/jpvol6Iss2pp102

Abstract

One of the potential agricultural products from Pasinan Village, Lekok sub-district, Pasuruan, East Java Province is Kawista Fruit. Residents of Pasinan used it for consumption, both raw and ripe. The large consumption of this fruit was caused an abundance of fruit peel. Based on the research results of phytochemical test, the extract of kawista fruit peel contains saponins, tannins, alkaloids, flavonoids, and terpenoids. Another income source of Pasinan residents comes from marine resources, that can be served in fresh fish, dried fish and other processed fish is susceptible to contaminated by microorganisms such as Salmonella sp. Salmonella sp. is pathogenic microbe lives on intestines of animals and humans. This study aims to determine the antibacterial activity of kawista peel extract on the growth of Salmonella sp. in-vitro and to determine the concentration of Kawista extract which is effective for inhibiting the growth of Salmonella sp using the paper disc diffusion method. The results of this study, the diameter of the inhibitory zone for the growth of Salmonella sp. using variables concentrations of kawista rind extract at 12.5%;25%;50%;75%;100%, with negative control are distilled water and methanol and positive control is tetracycline. At a concentration of 100% extract, the diameter of the bacterial inhibition test was the largest and significantly different from another treatment group at concentrations of 12.5%;25%;50%;75%. However, the 100% extract showed a smaller inhibitory zone than the tetracycline treatment group, but not significantly different. Based on the results of this research, it can be determined that kawista peel extract is effective for inhibited the growth of Salmonella sp. Kawista peel extract at a concentration of 100% is most effective for inhibiting the growth of Salmonella sp and shows results that are not significantly different from tetracycline.

Keywords: Antibacterial, Limonia acidissima L, Salmonella sp.

References

Brooks GF. et al. Medical Microbiology Textbook. Jakarta: Salemba Medika. 2005. 364-370

Cowan, M.M. 1999. Plant Product as Antimicrobial Agents. J. Microbiology Reviews. 12(4):564- 582.

Dyozem, J. P., Hamamoto, H., Ngameni, B., Ngadjui, B. T., dan Sekimizu, K. 2013. Antimicrobial Action Mechanism of Flavonoids from Dorstenia species. Drug Discoveries & Therapeutics, 7(2): 66-72.

Harborne, J. B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Institut Teknologi Bandung (Diterjemahkan oleh Kosasih Padmawinata dan Iwang Soediro)

Manik, D.F., Hertiani, T., dan Anshory, H. 2014. Analisis kolerasi antara kadar flavanoid dengan aktivitas antibakteri ekstrak etanol dan fraksi-fraksi daun kersen (Muntingia calaburam L.) terhadap Staphylococcus aurues. Khazanah, 6(2):1-11.

Mariajancyrani, J., Chandramohan, G., Saravanan, dan Elayaraja, A. 2013. Isolation and Antibacterial Activity of Terpenoid from Bougainvillea glabra Choicy Leaves. Asian Journal of Plant Science and Research, 3(3):70-73.

Mawan, A. R., Indriwati, S. E., & Suhadi.(2018). Aktivitas antibakteri ekstrak metanol buah Syzygium polyanthum terhadap pertumbuhan bakteri Escherchia coli. Bioeksperimen, 4(1), 64-68.

Mukhriani, Nurlina, Baso FF. 2014. Uji aktivitas antimikroba dan identifikasi ekstrak buah sawo manila (Achras zapota L.) terhadap beberapa mikroba patogen dengan metode difusi agar. JF FIK UINAM. 2(2):69-74.

Panda N, Patro VJ, Jena BK, Panda PK. 2013. Evaluation of phytochemical and anti-microbial activity of Limonia acidissima L. Int J Herbal Med. 2013; 1(1):22-27.

Pfoze, N. L., Kumar, Y., Myrboh, B., Bhagobaty, R. K., dan Joshi, S. R. 2011. In vitro Antibacterial Activity of Alkaloid Extract from Stem Bark of Mahonia manipurensis Takeda. Journal of Medicinal Plants Research, 5(5): 859-861.

Pui CF. Salmonella: A foodborne pathogen. International Food Research Journal. 2011. 18: 465-470

Radar Bromo. 2018. Artikel : Hasil laut melimpah dijadikan kerupuk ikan desa pasinan. Sabtu 8 september 2018

Ravi, L., Manasvi, V., dan Praveena, L.B. 2016. Antibacterial and antioxidant activity of saponin from Abutilon indicum leaves. Asian J Pharm Clin Res, 9:344-347.

Rini, dkk., 2017. Phytochemical Screening and Antibacterial Test of Ethanolic Extract of Kawista (Limonia Acidissima L.) From Aceh Besar Against Escherichia Coli. Jurnal Ilmiah Fakultas Keguruan dan Ilmu Pendidikan Unsyiah. 2(1), 12.

Schmeller, T., Latz-Brüning, B., dan Wink, M. 1997. Biochemical Activities of Berberine, Palmatine and Sanguinarine Mediating Chemical Defence Against Microorganisms and Herbivores. Phytochemistry. 44(2): 257-266.

Sukamto, L. A. 2000. Kultur Biji Kupas dan Tanpa Kupas Kawista Secara in Vitro. Dalam: Prosiding Seminar Nasional III. Universitas Lampung. Bandar Lampung. 160-163.

Sulastrianah., Imran, dan Fitria, E.S. 2014. Uji daya hambat ekstrak daun sirsak (Annona muricata L) dan daun sirih (Piper betle L) terhadap pertumbuhan bakteri Escherichia coli. Jurnal UHO, 1(1):76-84.

Susanti, N. 2016. Aktivitas antimikroba ekstrak rimpang jeringau terhadap pertumbuhan Candida albicans. Jurnal Biodjati, 1(1):55-58.

Taufiq, S., Umi, Y. dan Siti, H. 2015. Uji Aktivitas Antibakteri Ekstrak Etanol Biji Buah Pepaya (Carica papaya L.) terhadap Eschericia coli dan Salmonella typhi. Prosiding Penelitian Spesia Unisba. ISSN 2460-6472.

Wibowo, Muliana, dan Prabowo. 2010. Analisis Residu Antibiotik Kloramfenikol Dalam Daging Ikan Gurami (Osphronemus gouramy, Lac) Menggunakan Metode High Performance Liquid Chromatography. Jurnal Ilmiah Farmasi Volume 7 Nomor 1 Tahun 2010

Yuliana, S.R.I., Leman, M.A., dan Anindita, P.S. 2015. Uji daya hambat senyawa saponin batang pisang (Musa paradisiacal) terhadap pertumbuhan Candida albicans. Jurnal e-GiGi, 3(2):2.

Zhi-hui, Y., Xue-zhi, D., Li-qiu, X., Xiu-quing, X., Sha, X., Shuang, L., dan Xue-mei, L. 2013. Antimicrobial Activity and Mechanism of Total Saponins from Allium chinense. Food Science, 34(15): 75-80.