Eksplorasi Fungi Endofit dari Tanaman Sambung Nyawa (Gynura procumbens (Lour.)Merr.)

Ranti; Ummi Hiras  Habisukan; Amin  Nurokhman

doi:10.33541/jpvol6Iss2pp102

Ranti Universitas Islam Negeri Raden Fatah Palembang
Ummi Hiras Habisukan Universitas Islam Negeri Raden Fatah Palembang
Amin Nurokhman Universitas Islam Negeri Raden Fatah Palembang

DOI: https://doi.org/10.33541/jpvol6Iss2pp102

Keywords: Endophytic fungi, identification, isolation, sambung nyawa

Abstract

Sambung nyawa (Gynura procumbens(Lour.) Merr.) is a plant that can be used as herbal medicine such as healing cuts, lowering blood sugar levels, facilitating menstruation, and preventing cancer formation. The various properties possessed by sambung nyawa plants make these plants continue to be used and experience plant scarcity. Another alternative is to use endophytic fungi which are known as a source of efficacious medicinal secondary metabolites but have not been widely used. This study aims to isolate and identify the genus of endophytic fungi on sambung nyawa plants. The method used in this study was sampling, namely root organs, root bark, stems and leaves of sambung nyawa plants, making media using instant PDA media mixed with aquadest and antibiotics, endophytic fungi using direct planting techniques on PDA media in cups. petri, purification of endophytic fungi by eliminating fungi that are considered to be the same from their morphological characteristics until truly pure fungi are found, endophytic fungi help macroscopically by observing the color, texture, and pattern of the colony then observing microscopically using the culture slide technique then observing under a hyrox microscope by observing the type of spore, spore shape and hyphae. The results of this study were 12 isolates of endophytic fungi identified in 10 different genera, namely Nigrospora, Blastomyces, Fusarium, Pythium, Cladosporium, Trichoderma, Gliocladium, Papulaspora, Aspergillus, Monacrosporium.

References

Afzal, H., Shazad, S., Qomar, S., Nisa, U. (2013). Morphological identification of aspergillus species from the soil of larkana district (Sindh pakistan). Asian Journal of Agriculture and Biology, 1(3), 105–117.

Agustira, A., & Trijayanthi, W. (2019). Tanaman Sambung Nyawa(Gynura procumbens)Sebagai Antihiperglikemi. Jurnal Medula, 9(1), 1–5.

Akhsan, N.I.M, Sila, S., Syaifudin, E. A., dan Kurniati, I. R. A. (2022). Identifikasi jamur Rhizosfer di Lahan Tanaman Bawang Merah (Alliuma ascalonicum L) Bergulma di desa Bendang Raya Kecamatan Tenggarong. Jurnal Agroekoteknologi Tropika Lembab, 4(1), 99–106.

Akmalasari, I., Purwati, E. S., & Dewi, R. S. (2013). Isolasi dan identifikasi jamur endofit tanaman manggis (Garcinia mangostana L). Biosfera, 30(2), 82–89.

Andre, A. W. A. dan C. (2016). Molecular phyogeny and taxonomy of genus pythium. Mycological research. Mycological Research, 108(12), 1363–1383.

Aprilani, D., Mustofa, S., & Mutiara, U. G. (2019). Efek Pemberian Daun Sambung Nyawa ( Gynura procumbens ) Terhadap Kadar Gula Darah. Medical Journal Of Lampung University, 8(2), 305–308.

Ariyono, R.Q., S. D. and L. & S. (2014). Keanekaragaman Jamur Endofit Daun Kangkung Darat (Ipomoea reptans Poir.) pada Lahan Pertanian Organik dan Konvensional. Hama Dan Penyakit Tumbuhan, 2(1), 19–28.

Barnet, H.L & Hunter, B. . (1972). Illustrated genera of imperfect fungi (Third Edition). Minneapolis, Minnesota.

Fadli, M. Y. (2015). Benefits Of Sambung Nyawa (Gynura procumbens) Subtance As Anticancer. J Majority, 4(5), 50–53.

Fauziah, N., Noviyanti., dan Musthapa, I. (2018). The utilization of jambu bol (Syzigium malaccense) stem as a new source of antioxidants. Jurnal Ilmiah Farmaka Bahari, 10(1), 33–41.

Ghofur, A., Hamid, I.S., A. L. D. (2015). Anticarcinogenik activity of gynura procumbens extrac through cytochrome p450 and glutathione s-transerase. Int.J. PharmTech Res, 8, 24.

Habisukan, U. H., Elfita, Widjajanti, H., Setiawan, A., & Kurniawati, A. R. (2021). Diversity of endophytic fungi in syzygium aqueum. Biodiversitas, 22(3), 1129–1137.

Hapida, Y., Elfita E., Widjajanti, H., dan S. (2021). Biodiversity and antibacterial activity of endophytic fungi isolated from jambu bol (Syzygium malaccence). Jurnal Biodiversitas, 22(12), 5668–5677.

Hasyyati, N. S., Suprihadi, A., Raharjo, B., & Dwiatmi, K. (2017). Isolasi dan Karakterisasi Kapang Endofit dari Pegagan (Centella asiatica (L.) URBAN). Jurnal Biologi, 6(2), 66–74.

Hon, H. . (2009). No TThe genus pythium in taiwan, china (1). A Synoptic Review. Front, Biol China, 4(1), 15–28.

Imam, S., Cico, J.K.S., Dini, A. (2018). Penggunaan cendawan endofit sebagai agens pengendali hayati pada lada (Piper ningrum L.). Jurnal Agroqua, 16(2), 143–151.

Lestari, K., Agustien, A., & Djamaan, A. (2019). The Potential of Endophytic Fungi Isolated from Leaves, Stems, Mangrove Roots Avicennia marina as a Producer of Antibiotics. Metamorfosa: Journal of Biological Sciences, 6(1), 83–89.

Mamangkey, J, Mendes, LW, Harahap, A, Briggs, D, & Kayacilar, C. (2022a). Endophytic Bacteria and Fungi from Indonesian Medicinal Plants with Antibacterial, Pathogenic Antifungal and Extracellular Enzymes Activities: A Review. International Journal of Science, Technology & Management, 3(1), 245-255.

Mamangkey, J, Pardosi, L, & Wahyuningtyas, R, S. (2022b). Aktivitas Mikrobiologis Endofit dari Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Jendri. Jurnal Pendidikan Biologi Dan Ilmu Serumpun, 9(1), 376–386.

Melliawati, R. . H. (2009). Senyawa Antibakteri Escherichia coli ATCC 35218 dan Staphylococcus aureus ATCC 25923 dari Kapang Endofit Taman Nasional Gunung Halimun. Jurnal Natur Indonesia, 12(1), 21–27.

Misra, A.k., Gupta, V. (2009). Trichoderma:Biology, Biotechnology and biodiversity. J,Eco-Friendly Agric, 4(2), 99–117.

Mou, K. M., & Dash, P. R. (2016). A COMPREHENSIVE REVIEW ON GYNURA PROCUMBENS LEAVES. A Comprehensive Review on Gynura Procumbens, 3(4), 167–174. www.ijpjournal.com

Murdiyah, S. (2017). Endophytic fungi of various medicinal plants collected from evergreen forest Baluran national park and its potential as laboratory manual for mycology course. JPBI (Jurnal Pendidikan Biologi Indonesia), 3(1), 64–71.

Noverita, F. D. dan S. E. (2009). Isolasi dan Uji Aktivitas Antibakteri Jamur Endofit dari Daun dan Rimpang Zingiber Ottensi. Jurnal Farmasi Indonesia, 4(4), 172.

Novia, D., Lestari, G., & Malasari. (2022). Uji Efektivitas Ekstrak Etanol 96 % Daun Sambung Nyawa ( Gynura procumbens ( Lour ) Merr . ) Terhadap Luka Sayat Pada Kelinci Jantan ( Oryctolagus cuniculus ). Ilmiah Pharmacy, 9(1), 145–153.

Ozimek, E., Hanaka, A. (2021). Mortierella species as the plant growth promoting fungi present in the agriculture soils. Agriculture (Swizerland). Agriculture (Swizerland), 11(1), 1–8.

Pavan Kumar, G. V., & Naga Subrahmanyam, S. (2013). Phytochemical analysis, in-vitro screening for antimicrobial and anthelmintic activity of combined hydroalcoholic seed extracts of four selected folklore Indian medicinal plants. Der Pharmacia Lettre, 5(1), 168–176.

Putra, I. P. (2020). Ulasan: politik simbiosis fungi dan tumbuhan. Pro-Life, 7(2), 144–156. http://ejournal.uki.ac.id/index.php/prolife/article/view/1953

Rahmadini, A., & Fajar Utami, N. (2019). Uji Antibakteri Ekstrak Daun Sambung Nyawa (Gynura procumbens (Lour.) Merr.) dan Daun Tapak Liman (Elephantopus scaber L.) terhadap Salmonella thypi. Jurnal Ilmiah Ilmu Dasar Dan Lingkungan Hidup, 19(1), 1–11.

Rahmawati, E. (2017). Isolasi Dan Identifikasi Fungi Endofit Dari Buah Dan Daun Strawberry (Fragaria X Anannasa). Skripsi.UIN Malang.

Rahmi, S. (2019). Pemanfaatan ekstrak daun sambung nyawa ( Gynura procumbens ( Lour . Sebagai antipiretik pada tikus putih jantan. 1797–1803.

Ramadhani, S. H., Samingan, & I. (2017). Isolasi dan Identifikasi Jamur Endofit pada Daun Jamblang (Syzygium cumini L). Jurnal Ilmiah Mahasiswa Fakultas Keguruan Dan Ilmu Pendidikan Unsyiah, 2(2), 77–90.

Setiawan, M. A., Hasnawati, H., Sernita, S., & Sulistia, L. (2018). Uji Daya Hambat Antibakteri Fungi Endofit Kulit Jeruk Nipis (Citrus aurantifolia) Terhadap Bakteri Staphylococcus aureus. Jurnal Sains Farmasi & Klinis, 3(1), 14.

Shindy, C.I., Akhsan, N., S. (2020). Eksplorasi Jamur Nematofagus Dari Pupuk Kandang Di Kota Samarinda: Studi Kasus Kelurahan Lempake. Jurnal Agroekoteknologi Tropika Lembab, 3(1), 55–60.

Sopialena, S., Sopian, S., & Allita, L. D. (2019). Diversitas Jamur Endofit Pada Tanaman Padi (Oryza sativa L.) dan Potensinya Sebagai Pengendali Hama. Jurnal Agroekoteknologi Tropika Lembab, 2(1), 44–49.

Suhartina, Kandou, F. E. F., & Singkoh, M. F. O. (2018). Isolasi dan Identifikasi Jamur Endofit Pada Tumbuhan Paku Asplenium nidus. Jurnal MIPA, 7(2), 24.

Suliati, Rahmawati, & Mukarlina. (2017). Jenis- Jenis Jamur Endofit Tanaman Jeruk Siam ( Citrus nobilis var . microcarpa ) di Perkebunan Dungun Prapakan Sambas. Jurnal Protobiont, 6(3), 173–181.

Sulistiyono, F. D., & Mahyuni, S. (2019). Isolasi Dan Identifikasi Jamur Endofit Pada Umbi Talas (Colocasia es-culenta (L.) Schoot). Jurnal Sains Natural, 9(2), 66.

Sumadji, A. R., & Pitoyo, D. J. (2019). Potensi Pengembangan Tanaman Sambung Nyawa Sebagai Tanaman Obat Dan Pangan Skala Rumah Tangga. Prosiding, 223–227. https://pmat.unirow.ac.id/prosiding/index.php/SNasPPM/article/view/253

Suryandari, R. Y., Kasikoen, K. M., Martini, E., & Kurnianto, A. (2020). Pandemi Covid-19 Dan Kearifan Lokal: Penyuluhan Budidaya Tanaman Sambung Nyawa (Gynura Procumbens) Di Dusun Jomblang, Tegaltirto, Berbah, Sleman, Daerah Istimewa Yogyakarta. Anoa : Jurnal Pengabdian Masyarakat Sosial, Politik, Budaya, Hukum. Ekonomi, 1(3), 167–177.

Tan, H. L., Chan, K. G., Pusparajah, P., Lee, L. H., & Goh, B. H. (2016). Gynura procumbens: An overview of the biological activities. Frontiers in Pharmacology, 7(52), 1–14.

Utami, P., and Puspaningtyas, D, E. (2013). The Miracle of Herbs. PT Agromedia Pustaka.

Uthia, R., Kardela, W., & Transida, K. B. (2018). Uji Efek Antiinflamasi Ekstrak Etanol Daun Sambung Nyawa ( Gynura procumbens ( Lour .) Merr .) Terhadap Kaki Tikus Putih Jantan. Farmasi Higea, 10(1), 25–32.

Watanabe, T. (1937). Pictorial atlas of soil and seed fungi morphologies of cultured fungi and key to species.

Watanabe, T. (2010). Pictorial Atlas Of Soil And Seed Fungi Pictorial Atlas Of Soil And Seed Fungi Morphologies Of Cultures Fungi. CRC Press.

Wulandari, D., Liliek, S., dan A. M. (2014). Keanekaragaman Jamur Endofit Pada Tanaman Tomat (Lycopersicum esculentum Mill.) Dan Kemampuan Antagonisnya Terhadap Phytophthora infestans. Jurnal HPT, 2(1).

WuLandari. (2016). Potensi berbagai macam ekstrak tumbuhan sebagai antifungi dalam memperpanjang masa penyimpanan serta mempertahankan kualitas jagung. SKRIPSI Fakultas Perternakan Universitas Andalas Padang.

Y, H. (2020). Pengaruh Pemberian Rebusan Daun Sambung Nyawa (Gynura pro-cumbens) Terhadap Penurunan Tekanan Darah Pada Penderita Hipertensi.

Yuricha, K., Liliek, S., Abdul, C. (2015). Potensi Antagonisme Jamur Endofit Pada Tanaman Lada (Piper Ningrum L). Terhadap Jamur Phythophora Capsici Leioniam Penyebab Penyakit Busuk Pangkal Batang. Jurnal HPT, 3(1).