Aktivitas Mikrobiologis Endofit dari Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis)
Abstract
Endophytic bacteria and fungi inhibite the tisues of living plant. Both of them are significant components of plant microecosystems. Current research was performed to explain several microbiological activities (production of extracellular enzymes, antioxidants, and antibacterials) of endophytic bacteria and fungi from binahong leaves extract (Anredera cordifolia (Ten.) Steenis). In this case, the research was conducted by applying a review of several literature reviews published online on Google Scholar and several journals listed on the ScienceDirect site. Based on the results obtained from the research reports from several scientific journal sources, there are four types of extracellular enzymes produced by the endophytic bacteria of the Anredera cordifolia plant, including proteinase, amylase, esterase, and cellulase. In addition, the endophytic bacteria of Pseudomonas aeruginosa from the leaves extract of Anredera cordifolia has antioxidant activity, which is volatile compounds including cis-9-Octadecenoic acid, 2-dodecenoic acid, and 9 octadecenamide produced by endophytic bacteria of Pseudomonas sp. Furthermore, the endophytic bacteria isolated from Anredera cordifolia leaves extract inhibits the growth of E. coli ATCC 35216 and S. aureus ATCC 29523. Meanwhile, the endophytic fungi from Anredera cordifolia leaves extract inhibite the growth of E.coli and S. aureus bacteria. Therefore, understanding the relationship between bacteria and endophytic fungi from the leaves extract of binahong (Anredera cordifolia (Ten.) Steenis) is essential. This knowledge can be utilized and applied well for the future production of higher quality and more medicinal plants from binahong plant (Anredera cordifolia (Ten.) Steenis).
References
Abou-Elela GM, H Abd-Elnaby, HA Ibrahim, MA Okbah. 2009. Marine natural products and their potential applications as anti-infective agents. World Appl Sci J, 7(7):872–80.
Barman D & K Bhattacharjee. 2020. Chapter 8 Endophytic bacteria associated with medicinal plants: the treasure trove of antimicrobial compounds, in Medically Important Plant Biomes, eds D. Egamberdieva and A. Tiezzi (Singapore: Springer), 153–187.
Bhoonobtong A, S Sawadsitang, S Sodngam, W Mongkolthanaruk. 2012. Characterization of endophytic bacteria, Bacillus amyloliquefaciens for antimicrobial agents production. In: International Conference on Biological and Life Sciences, p. 6–11.
Bilal S, L Ali, AL Khan, R Shahzad, S Asaf, M Imran, SM Kang, SK Kim, IJ Lee. 2018. Endophytic fungus Paecilomyces formosus LHL10 produces sester-terpenoid YW3548 and cyclicpeptide that inhibit urease and α-glucosidase enzyme activities. Arch Microbiol, 200:1493–502.
Boyoma FF, RMK Toghueo, I Zabalgogeazcoa, BR Vázquez de Aldanab. 2017. Enzymatic activity of endophytic fungi from the medicinal plants Terminalia catappa, Terminalia mantaly and Cananga odorata. South Afr J Botany, 109:146–53
Brader G, S. Compant, B Mitter, F Trognitz, & A Sessitsch. 2014. Metabolic potential of endophytic bacteria. Curr. Opin. Biotechnol, 27: 30–37.
Buatong J, S Phongpaichit, V Rukachaisirikul, J Sakayaroj. 2011. Antimicrobial activity of crude extracts from mangrove fungal endophytes. World J Microbiol Biotechnol, 27(12):3005–3008.
Bundale S, D Begde, N Nashikkar, T Kadam, S Bundale, D Begde, N Nashikkar, T Kadam, A Upadhyay. 2015. Optimization of culture conditions for production of bioactive metabolites by Streptomyces spp. isolated from soil. Advances Microbiol, 5(06):441.
Castro RA, MC Quecine, PT Lacava, BD Batista, DM Luvizotto, J Marcon, A Ferreira, IS Melo, JL Azevedo. 2014. Isolation and enzyme bioprospection of endophytic bacteria associated with plants of Brazilian mangrove ecosystem. SpringerPlus, 3(1):382.
Darsana, IG Oka, INK Besung, H Mahatmi. 2012. Potensi Daun Binahong (Anredera cordifolia (Tenore) Steenis) dalam menghambat pertumbuhan bakteri Escherichia coli secara in vitro. Indonesia Medicus Veterinus, 1(3): 337-351.
Ek-Ramos MJ, R Gomez-Flores, AA Orozco-Flores, C Rodriguez-Padilla, G Gonzalez-Ochoa, & P Tamez-Guerra. 2019. Bioactive products from plant-endophytic gram-positive bacteria. Front. Microbiol. 10:463.
El-Deeb B, S Bazaid, Y Gherbawy, H Elhariry. 2012. Characterization of endophytic bacteria associated with rose plant (Rosa damascena trigintipeta) during flowering stage and their plant growth promoting traits. J Plant Interact, 7(3):248–53
Firáková S, M Šturdíková, & M Múˇcková. 2007. Bioactive secondary metabolites produced by microorganisms associated with plants. Biologia, 62: 251–257.
Garmana AN, EY Sukandar, I Fidrianny. 2014. Activity of several plant extracts against drug- sensitive and drug-resistant microbes. Procedia Chemistry, 13:164–169.
Ismail, Megawati, NF Bakri. 2018. Journal of Pharmaceutical and Medicinal Sciences 2018 3(2): pp 22-27
Istyastono EP, SH Yuliani. 2016. Scarless wound healing gel with Binahong (Anredera cordifolia (Ten) Steenis) leaves extract and celecoxib as the active ingredients. In: AIP Conference Proceedings: AIP Publishing, 160001.
Kandel SL, P Joubert, & S Doty. 2017. Bacterial endophyte colonization and distribution within plants. Microorganisms, 5(4):77.
Köberl M, R Schmidt, E Ramadan, R Bauer, & G Berg. 2013. The microbiome of medicinal plants: diversity and importance for plant growth. Front. Microbiol, 4:400.
Maggini V, M De Leo, A Mengoni, E Gallo, E Miceli, R Reidel. 2017. Plant-endophytes interaction influences the secondary metabolism in Echinacea purpurea (L.) Moench: an in vitro model. Sci. Rep. 7:16924.
Manoi F. 2009. Binahong (Anredera cordifolia) (Ten) Steenis) sebagai obat. Jurnal Warta Penelitian Dan Pengembangan Tanaman Industri. 15(1): 3.
Mardiana L. 2012. Daun ajaib tumpas penyakit. (Cetakan ke 1).Jakarta : Penebar swadaya Mokodompit
Monnanda SN, SP Harischandra, & VT Mysore. 2020. Chapter 13 Bioactive potentials of novel molecules from the endophytes of medicinal plants, in Medically Important Plant Biomes, eds D. Egamberdieva and A. Tiezzi (Singapore: Springer), 293–355.
Newman DJ. 2018. Are microbial endophytes the actual producers of bioactive antitumor agents. Trends Cancer, 4(10):662–70.
Nursulistyarini F, EQ Ainy. 2014. Isolasi dan Identifikasi Bakteri Endofit Penghasil Antibakteri dari Daun Tanaman Binahong (Anredera cordifolia (Ten.) Steenis). Proceeding Biology Education Conference, 11(1): 114-120
Nxumalo CI, LS Ngidi, JSE Shandu, TS Maliehe. 2020. Isolation of endophytic bacteria from the leaves of Anredera cordifolia CIX1 for metabolites and their biological activities. BMC Complementary Medicine and Therapies, 20:300
Özçelik B, M Aslan, I Orhan, T Karaoglu. 2005. Antibacterial, antifungal, and antiviral activities of the lipophylic extracts of Pistacia vera. Microbiol Res, 160(2):159–64.
Pinski A, A Betekhtin, K Hupert-Kocurek, L Mur, & R Hasterok. 2019. Defining the genetic basis of plant–endophytic bacteria interactions. Int. J. Mol. Sci. 20(8):1947
Prakash A. 2001.Antioxidant Activity. Medallion Laboratories: AnalithycalProgres, 19(2):1-4.
Prihatiningtiyas W & MSH Wahyuningsih. 2011. Prospek Mikroba Endofit Sebagai Sumber Senyawa Bioaktif .Artikel.http://mot.farmasi.ugm.ac.id/artikel-55-prospek-mikroba-endofit sebagaisumber-senyawa-bioaktif.html quality and health.
Rat A, HD Naranjo, N Krigas, K Grigoriadou, E Maloupa, AV Alonso, C Schneider, VP Papageorgiou, AN Assimopoulou, N Tsafantakis, N Fokialakis and A Willems. 2021. Endophytic Bacteria From the Roots of the Medicinal Plant Alkanna tinctoria Tausch (Boraginaceae): Exploration of Plant Growth Promoting Properties and Potential Role in the Production of Plant Secondary Metabolites. Frontiers in Microbiology, 12: 633488.
Rodriguez RJ, JF White, AE Arnold, & RS Redman. 2009. Fungal endophytes: diversity and functional roles. NewPhytol. 182: 314–330.
Rofida S. 2010. Studi Etnobotani Dan Etnofarmakologi Umbi Binahong (Anredera cordifolia (Ten) Steenis). Farmasains : Jurnal Farmasi dan Ilmu Kesehatan, 1(1).
Setiaji A. 2009. Uji Aktivitas Antibakteri Ekstrak Petroleum Eter, Etil Asetat Dan Etanol 70% Rhizoma Binahong (Anredera cordifolia (Tenore) Steenis) Terhadap Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 11229 Serta Skrining Fitokimianya. Skripsi Tidak Diterbitkan. Surakarta: Fakultas Farmasi UMS Surakarta.
Sidabutar R. 2018. Uji aktivitas antibakteri ekstrak etanol daun binahong (Anredera cordifolia (Ten.) Steenis) terhadap Staphylococcus aureus dan Salmonella Typhi dengan metode difusi agar. Skripsi.
Singh M, A Kumar, R Singh, KD Pandey. 2017. Endophytic bacteria: A new source of bioactive compounds. 3 Biotech, 7(5):315.
Strobel G, B Daisy. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews, 67(4); 491–502.
Susetya D. 2012. Khasiat dan manfaat daun ajaib Binahong.(Cetakan ke 1)Yogyakarta : Pustaka Baru Press.
Tan RX, WX Zou. 2001. Endophytes: A rich source of functional metabolites. Natural Product Reports, 18(4): 448–459.
Veronita F, N Wijayati, S Mursiti. 2017. Isolasi dan uji aktivitas antibakteri daun binahong serta aplikasinya sebagai hand sanitizer. Indonesian Journal of Chemical Science, 6(2); 138–144
Wu W, W Chen, S Liu, J Wu, Y Zhu, L Qin, B Zhu. 2021. Beneficial Relationships Between Endophytic Bacteria and Medicinal Plants. Frontiers in Plant Science, 12: 646146
Zhang ZP, CC Shen, FL Gao, H Wei, DF Ren, J Lu. 2017. Isolation, purification and structural characterization of two novel water-soluble polysaccharides from Anredera cordifolia. Molecules, 22:1276.
Zhang HW, YC Song, RX Tan. 2006. Biology and chemistry of endophytes. Nat.Prod.Rep. 23: 753–771.
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