Pemberian Asam Humat untuk Meningkatkan Pertumbuhan dan Kandungan Total Flavonoid Sawi Hijau (Brassica juncea L.) pada Perbedaan Kadar Salinitas
Abstract
Salt affects plant growth due to increased soil osmotic pressure and plant nutrients disturbance. Most plants are susceptible to high salinity which can cause physiological and biochemical interference. This research was conducted to obtain a possible salinity level for mustard greens (Brassica juncea L.) to grow well and the best humic acid dosage to reduce salinity damages and to gain high total flavonoid content. This research used Randomized Block Design method with two factors and four replications. The factors are salinity levels (control, 50, 75, and 100 mM) and humic acid dosage (control, 2, 4, and 8 g/kg). In the parameters which had interactions between the two treatments, the highest results were obtained with a combination of 100 mM salinity and 12 g humic acid, such as plant height (33.35 cm), leaf area (379.66 cm2), fresh weight (22.41 g) and dry weight (1.54 g). The highest results on leaf number (8.5 leaves) and total flavonoid content (0.074 mgQE/g sample) were obtained with 100 mM salinity. Humic acid dose of 12 g gave the highest result on leaf number (8.69 leaves) and total flavonoid content (0.095 mgQE/g sample).
References
Abogadallah GM. 2010. Insights into the significance of antioxidative defense under salt stress. Plant Signaling & Behavior 5(4): 369–374.
Adetya V, S Nurhatika & A Muhibuddin. 2018. Pengaruh pupuk mikoriza terhadap pertumbuhan cabai rawit (Capsicum frutescens) di tanah pasir. Jurnal Sains Dan Seni ITS 7(2): 2337–3520.
Ali AYA, MEH Ibrahim, G Zhou, NEA Nimir, X Jiao, G Zhu, AMI Elsiddig, MSE Suliman, SBM Elradi & W Yue. 2020. Exogenous jasmonic acid and humic acid increased salinity tolerance of sorghum. Agronomy Journal 112(2): 871-884.
Ali MB, N Singh, AM Shohael, EJ Hahn, & KY Paek. 2006. Phenolics metabolism and lignin synthesis in root suspension cultures of Panax ginseng in response to copper stress. Plant Science, 171(1): 147–154.
Bai X, L Dai, H Sun, M Chen, & Y Sun. 2019. Effects of moderate soil salinity on osmotic adjustment and energy strategy in soybean under drought stress. Plant Physiology and Biochemistry, 139(2): 307-313.
Bakry BA, MH Taha, ZA Abdelgawad, & MMS Abdallah. 2014. The role of humic acid and proline on growth, chemical constituents and yield quantity and quality of three flax cultivars grown under saline soil conditions. Agricultural Sciences, 5(14):1566-1575.
Bengough AG, BM McKenzie, PD Hallett, & TA Valentine. 2011. Root elongation, water stress, and mechanical impedance: a review of limiting stresses and beneficial root tip traits. Journal of Experimental Botany, 62(1): 59-68.
Canellas LP, NOA Canellas, da LES Irineu, FL Olivares, & A Piccolo. 2020. Plant chemical priming by humic acids. Chemical and Biological Technologies in Agriculture, 7(1): 1-17.
Chang CC, MH Yang, HM Wen, & JC Chern. 2002. Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis, 10(3): 178-182.
DaurDaur I, & AAS Bakhashwain. 2013. Effect of humic acid on growth and quality of maize fodder production. Pakistan Journal of Botany, 45: 21–25.
El-Hefny EM. 2010. Effect of saline irrigation water and humic acid application on growth and productivity of two cultivars of cowpea (Vigna unguiculata L. Walp). Australian Journal of Basic and Applied Sciences, 4(12): 6154–6168.
El-Sarkassy NM, SA Ibrahim, & EM Desoky. 2017. Salinity stress amelioration using humic acid and mycorrhizae on pepper plants. Zagazig Journal of Agricultural Research, 44(6): 2515- 2527.
Fauziah I, E Proklamasiningsih, & I Budisantoso. 2019. Pengaruh asam humat pada media tanam zeolit terhadap pertumbuhan dan kandungan vitamin C sawi hijau (Brassica juncea). BioEksakta: Jurnal Ilmiah Biologi Unsoed, 1(2): 17-21.
Hadid A, P Sarif, & W Imam. 2015. Pertumbuhan dan Hasil Tanaman Sawi (Brassica juncea L.) Akibat pemberian berbagai dosis pupuk urea. Agrotekbis, 3(5): 585–591.
Haider N. 2017. Influence of humic acid application on phenology, leaf area and production duration of okra (Abelmoschus esculentus L.) cultivars. Pure and Applied Biology, 6(3): 1010-1020.
Hoang HL, CC de Guzman, NM Cadiz, & DH Tran. 2020. Physiological and phytochemical responses of red amaranth (Amaranthus tricolor L.) and green amaranth (Amaranthus dubius L.) to different salinity levels. Legume Research-An International Journal, 43(2): 206–211.
Istiqomah, & AD Serdani. 2018. Pertumbuhan dan hasil tanaman sawi (Brassica juncea L. Var. Tosakan) pada pemupukan organik, anorganik dan kombinasinya. Agroradix, 1(2): 1–8.
Jamil M, CC Lee, SU Rehman, DB Lee, M Ashraf, & ES Rha. 2005. Salinity (NaCl) tolerance of Brassica species at germination and early seedling growth. Electronic Journal of Environmental, Agricultural and Food Chemistry, 4(4), 970–976.
Javanshah A & SA Nasab. 2017. The Effects of humic acid and calcium on morpho-physiological traits and mineral nutrient uptake of pistachio seedling under salinity stress. Journal of Nuts, 7(2): 125–135.
Katerji N, JW van Hoorn, A Hamdy, M Mastrorilli, & EM Karzel. 1997. Osmotic adjustment of sugar beets in response to soil salinity and its influence on stomatal conductance, growth and yield. Agricultural Water Management, 34(1): 57-69.
Kementrian Pertanian. 2013. Data lima tahun subsector hortikultura. http://www.deptan.go.id/infoeksekutif/horti/isi/_dt5thn_horti.php
Khaled H & HA Fawy. 2011. Effect of different levels of humic acids on the nutrient content, plant growth, and soil properties under conditions of salinity. Soil and Water Research, 6(1): 21–29.
Kim HJ, JM Fonsesca, JH Choi, C Kubota, & D Kwon. 2008. Salt in irrigation water affects the nutritional and visual properties of romaine lettuce (Lactuca sativa L.). Journal of Agricultural and Food Chemistry, 56(10): 3772–3776.
Kim YH, AL Khan, ZK Shinwari, DH Kim, M Waqas, M Kamran, & IJ Lee. 2012. Silicon treatment to rice (Oryza sativa l. cv ’gopumbyeo’) plants during different growth periods and its effects on growth and grain yield. Pakistan Journal of Botany, 44(3): 891–897.
Lumbessy M, J Abidjulu, & JJE Paendong. 2013. Uji total flavonoid pada beberapa tanaman obat tradisonal di desa Waitina Kecamatan Mangoli Timur Kabupaten Kepulauan Sula Provinsi Maluku Utara. Jurnal MIPA, 2(1): 50-55.
Machado RMA & RP Serralheiro. 2017. Soil salinity: effect on vegetable crop growth. Management practices to prevent and mitigate soil salinization. Horticulturae, 3(2): 1-13.
Martajaya M. 2018. Pertumbuhan dan hasil jagung manis (Zea mays Saccharata Stury) yang dipupuk dengan pupuk organik dan anorganik pada saat yang berbeda. Jurnal Ilmiah Budidaya, 2(2): 90–102.
Mazher AMA, EMF El-Quesni, & MM Farahat. 2007. Responses of ornamental and woody trees to salinity. World J. Agric. Sci, 3(3): 386–395.
Memon SA, XL Hou, & LJ Wang. 2010. Morphlogical analysis of salt stress response of pak choi. Electronic Journal of Environmental, Agricultural & Food Chemistry, 9(1): 248–254.
Nurmayulis U, P Utama, & R Jannah. 2018. Pertumbuhan dan hasil tanaman selada (Lactuca sativa) yang diberi bahan organik kotoran ayam ditambah beberapa bioaktivator. Agrologia, 3(1).
Proklamasiningsih E, I Budisantoso, & I Maula. 2019. Pertumbuhan dan kandungan polifenol tanaman katuk (Sauropus androgynus (l.) Merr) pada media tanam dengan pemberian asam humat. Al-Kauniyah: Jurnal Biologi, 12(1): 96–102.
Rachman A, IGM Subiksa, & Wahyunto. 2007. Perluasan areal tanaman kedelai ke lahan suboptimal. http://balitkabi.litbang.pertanian.go.id/wp-content/uploads/2016/03/dele_8.rachman-1.pdf
Siddiqui MH, F Mohammad, & MN Khan. 2009. Morphological and physio-biochemical characterization of Brassica juncea L. Czern. & Coss. genotypes under salt stress. Journal of Plant Interactions, 4(1): 67–80.
Soedradjad R & A Syamsunihar. 2017. Kandungan fenolik dan flavonoid biji tanaman kedelai yang berasosiasi dengan Synechococcus sp. dan dipupuk organik. Agritrop Jurnal Ilmu-Ilmu Pertanian, 12(1): 5–8.
Studer C, Y Hu, & U Schmidhalter. 2007. Evaluation of the differential osmotic adjustments between roots and leaves of maize seedlings with single or combined NPK-nutrient supply. Functional Plant Biology, 34(3): 228–236.
Syakir M, MN Maslahah, & Januwati. 2008. Pengaruh salinitas terhadap pertumbuhan, produksi dan mutu sambiloto (Andrographis paniculata Nees). Buletin Penelitian Tanaman Rempah Dan Obat (BUL LITTRO), 19(2): 129–137.
Tavakkoli E, F Fatehi, S Coventry, P Rengasamy, & GK McDonald. 2011. Additive effects of Na+ and Cl– ions on barley growth under salinity stress. Journal of Experimental Botany, 62(6): 2189–2203.
Turan MA, AH Awadelkarim, & A Elkarim. 2009. Effect of salt stress on growth, stomatal resistance, proline and chlorophyll concentrations on maize plant. African Journal of Agricultural Research, 4(9): 893–897.
Walker DJ & MP Bernal. 2004. Plant mineral nutrition and growth in a saline Mediterranean soil amended with organic wastes. Communications in Soil Science and Plant Analysis, 35, 2495–2514.
Yao LH, YM Jiang, J Shi , FA Tomas-Barberan, N Datta, R Singanusong, & SS Chen. 2004. Flavonoids in food and their health benefits. Plant Foods for Human Nutrition, 59(3): 113-122.
Zaremanesh H, H Eisvand, A Ismaili, & N Akbari. 2019. Effects of different humic acid and salinity levels on some traits of khuzestani savory (Satureja Khuzistanica Jamzad). Applied Ecology and Environmental Research, 17(3): 5409-5433.
Zhai Y, Q Yang & M Hou. 2015. The effects of saline water drip irrigation on tomato yield, quality, and blossom-end rot incidence A 3a case study in the South of China. PLOS ONE, 10(11): e0142204.
Zhou L, NN Wang, SY Gong, R Lu, Y Li, & XB Li. 2015. Overexpression of a cotton (Gossypium hirsutum) WRKY gene, GhWRKY34, in Arabidopsis enhances salt-tolerance of the transgenic plants. Plant Physiology and Biochemistry, 96: 311-320.
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