Isolasi Kurkumin dari Kunyit Putih dengan Menggunakan Metode Maserasi dan Kromatografi Lapis Tipis (KLT)
Abstract
Curcumin is an active compound of the polyphenol group found in turmeric. Isolation and identification of curcumin have been done in plants which belong to genus Curcuma, such as Curcuma zedoaria. Curcumin is known for its antitumor, antioxidant properties and has many health benefits. Curcumin have many therapeutic activities, such as antioxidant, anti-inflammatory, and anticarcinogenic. The isolation and identification process was used maceration and TLC (Thin Layer Cromatography) method. The reason using this methods are efficient, easy, simple, and cheap. The extract characterization gave the water content valued at 0,37%, total ash valued at 0,7%, the acid insoluble ash valued at 0,291%. All of these extract characterization adequate the provision. From the results of TLC with a mixture of mobile phase (eluent) i.e. chloroform:ethanol:acetic acid with a ratio of 94:5:1, it shows that there are 3 stains with Rf values, respectively, for curcumin standard Rf1 0.1-0.125; Rf2 0.1375-0.1875; Rf3 0.3375-0.375. As for the white turmeric extract obtained Rf1 0.125-0.225; Rf2 0.1875-0.475; Rf3 0.425-0.675. Meanwhile, the mobile phase mixture (eluent) of chloroform:n-hexane:methanol with a ratio of 1:1:0,1 showed that there were 3 stains with Rf values, respectively, for curcumin the standard was Rf1 0.1-0.125; Rf2 0.1375-0.1875; Rf3 0.3375-0.375. As for the white turmeric extract obtained Rf1 0.1-0.125; Rf2 0.125-0.225; Rf3 0.35-0.375.
References
Alen Y, FL Agresa, & Y Yuliandra. 2017. Analisis Kromatografi Lapis Tipis (KLT) dan Aktivitas Antihiperurisemia Ekstrak Rebung Schizostachyum Brachycladum Kurz (Kurz) Pada Mencit Putih Jantan. Jurnal Sains Farmasi & Klinis, 3(2): 148.
Chairunnisa S, NM Wartini, & L Suhendra. 2019. Pengaruh Suhu Dan Waktu Maserasi Terhadap Karakteristik Ekstrak Daun Bidara (Ziziphus Mauritiana L.) Sebagai Sumber Saponin. Jurnal Rekayasa Dan Manajemen Agroindustri, 7(4): 552.
Damayanti A, & EA Fitriana. 2012. Pemungutan Minyak Atsiri Mawar (Rose Oil) Dengan Metode Maserasi. Jurnal Bahan Alam Terbarukan, 1(2): 3.
Daulay AS, & S Nadia. 2019. Eksplorasi Ekstrak Kurkuminoid Rimpang Kunyit Dengan Perbandingan Metode Maserasi Dan Pelarut Berdasarkan Aktivitas Antioksidan. Prosiding Seminar Nasional & Expo II Hasil Penelitian Dan Pengabdian Masyarakat, Halaman 1723.
Febriawan R. 2020. Manfaat Senyawa Kurkumin Dalam Kunyit Pada Pasien Diare. Jurnal Medika Hutama, 2(1): 257.
Kartikasari D, Nurkhasanah, & S Pramono. 2014. Karakterisasi Simplisia Dan Ekstrak Etanol Daun Bertoni (Stevia rebaudiana) Dari Tiga Tempat Tumbuh. Jurnal Ilmu Farmasi dan Farmasi Klinik, 1(1): 149-150.
Kementrian Kesehatan RI. 2017. Farmakope Herbal Indonesia. Edisi II. Jakarta: Kementrian Kesehatan RI. Halaman 526-527.
Loboa R, KS Prabhua, A Shirwaikara, & A Shirwaikarb. 2009. Curcuma zedoaria Rosc. (white turmeric): a review of its chemical, pharmacological and ethnomedicinal properties. Journal of Pharmacy and Pharmacology, 61: 14.
Namir H, R Hadžić, I Malešević, M Jurčević, & D Starčević. 2019. Application of thin layer chromatography for qualitative analysis of gunpowder in purpose of life prediction of ammunition. International Journal of Biosensors & Bioelectronics, 5(1): 5-6.
Puspitasari AD, & LS Proyogo. 2017. Perbandingan Metode Ekstraksi Maserasi Dan Sokletasi Terhadap Kadar Fenolik Total Ekstrak Etanol Daun Kersen (Muntingia Calabura). Jurnal Ilmiah Cendekia Eksakta. 2(1): 2.
Suharsanti R, C Astutiningsih, & ND Susilowati. 2020. Kadar Kurkumin Ekstrak Rimpang Kunyit (Curcuma Domestica) Secara KLT Densitometri Dengan Perbedaan Metode Ekstraksi. Journal Wiyata, 7(2): 86.
- View 9610 times Download 9610 times PDF
Copyright (c) 2021 Henni Cintya, Michelle Angie Chan, Anggreni Purba, Tanezsia Kokita, Farrencia Destinyie, Wilbert Bernardi
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
