INDUKSI KALUS KRISAN (Chrysanthemum morifolium Ramat var. Tomohon Kuning) DENGAN 2,4-Dichlorophenoxyacetic Acid (2,4-D) DAN 6-Benzylaminopurine (BAP) PADA KONDISI PENCAHAYAAN BERBEDA

Abstract

This study aims to obtain the optimum concentration of 2,4-D and BAP plant growth regulators (PGRs) to induce
Chrysanthemum callus in light and dark conditions. The method used is an experimental method in the laboratory
using a Completely Randomized Design (CRD) with 4 treatment of 2,4-D + BAP concentration combinations. The
culture was incubated under different lighting conditions for 45 days after planting. The parameters observed
included ctexture and color of callus, other responses produced by explants, size, fresh weight and dry weight of
callus. Data were analyzed descriptively. The results showed that 4 ppm 2,4-D + 0.5 ppm BAP treatment was the
best combination in inducing Chrysanthemum callus in both light and dark conditions. In bright conditions, most
of the callus were dark green and dark brown with a compact texture, callus size of 1.36 cm, and the highest fresh
weight and dry weight of callus were 0.62 gram and 0.17 gram respectively. Meanwhile, in the dark conditions
most of the callus were light green and light brown with a compact texture, callus size 1.18 cm, and the highest
fresh weight and dry weight of the callus produced were 0.51 grams and 0.15 grams, respectively.

Keywords: Callus, Chrysanthemum morifolium, 2,4-Dichlorophenoxyacetic acid (2,4-D), 6-Benzylaminopurine
(BAP)