Efek Antimikroba Infusa Biji Pinang (Areca catechu Linn.), Daun Sirih (Piper betle Linn) dan Kombinasinya Terhadap Staphylococcus aureus Secara In Vitro

Abstract

Abstrak
Staphylococcus aureus menjadi bakteri penyebab utama terjadinya abses di rongga mulut. Frekuensi dental abses
di dunia mulai dari 0,7% sampai 15% dan 14% di Indonesia. Biji pinang (Areca catechu L.) dan Daun sirih (Piper
betle L.) memiliki aktivitas antimikroba. Tujuan penelitian ini adalah menilai aktivitas antimikroba dari infusa
biji pinang, daun sirih dan kombinasinya terhadap Staphylococcus aureus. Penelitian ini bersifat eksperimental
laboratorik metode difusi dengan metode Kirby-Bauer. Penelitian ini dilakukan triplo. Rerata zona inhibisi terbesar
terdapat pada ciprofloxacin (25,46±0,19 mm), diikuti infusa biji pinang 6% (11,76±0,96 mm), 8% (11,60±0,64
mm), 10% (11,38±0,96 mm), kombinasi infusa biji pinang 6% dan daun sirih (10,88±0,72mm), infusa daun sirih
(9,82±0,81 mm), kombinasi infusa biji pinang 8% dan daun sirih (8,66±0,41 mm), dan kombinasi infusa biji pinang
10% dan daun sirih (8,54±1,23 mm). Simpulan penelitian infusa biji pinang 6%, 8%, 10%, infusa daun sirih, dan
kombinasinya memiliki efek antimikroba terhadap S. aureus.
Kata Kunci: biji pinang; daun sirih; antimikroba; Staphylococcus aureus

Abstract
Staphylococcus aureus is the main cause of abscesses in the oral cavity. The frequency of dental abscesses in the
world ranges from 0.7% to 15% and 14% in Indonesia. Areca nut (Areca catechu L.) and Betel leaf (Piper betle L.)
has antimicrobial activity. The purpose of this study was to measure the inhibition zone from infusion of areca nut,
betel leaf and their combination against Staphylococcus aureus and is expected to be a complementary therapy. This
research is a laboratory experimental study using the Kirby-Bauer method of diffusion. This research was conducted
in triplo. The largest mean zone of inhibition was found in ciprofloxacin (25.46±0.19 mm), followed by areca nut
infusion 6% (11.76±0.96 mm), 8% (11.60±0.64 mm), 10% (11.38±0.96 mm), combination of betel leaf infusion
and 6% areca nut (10.88±0.72mm), betel leaf infusion (9.82±0.81 mm), combination of betel leaf and 8% areca nut
(8.66±0.41 mm), and combination of 10% areca nut infusion and betel leaf (8.54±1.23 mm). The conclusion of this
research was that betel nut infusion 6%, 8%, 10%, betel leaf infusion, and their combination have antimicrobial
effect against S.aureus.
Keywords: areca nut; betel leaf; antimicrobial; Staphylococcus aureus
*DR: Penulis Koresponden; Email: jayarusmana67@gmail.com