Pengujian Infusa Rebusan Bunga Telang (Clitoria ternatea) Sebagai Pewarna Alami Sediaan Apus Darah Tepi (SADT)

Fajar Husen; Nur Aini Hidayah Khasanah

doi:10.33541/edumatsains.v8i1.4419

Fajar Husen Sekolah Tinggi Ilmu Kesehatan (STIKes) Bina Cipta Husada
Nur Aini Hidayah Khasanah Sekolah Tinggi Ilmu Kesehatan (STIKes) Bina Cipta Husada Purwokerto

DOI: https://doi.org/10.33541/edumatsains.v8i1.4419

Abstract

Telang flower (Clitorea ternatea) is a flower that belongs to the endemic plants that are often found in Ternate. C. ternatea belongs to the Fabaceae family of leguminous plants and is the world's most prominent family of flowering plants. C. ternatea contains a lot of blue pigments or cyanos known as anthocyanins. These dyes have great potential as alternative dyes to replace methylene blue and are potential blood cell dyes. This study aimed to examine the potential of the anthocyanin content in the butterfly pea flower infusion as a natural differential dye for erythrocytes and leukocytes. This research method is descriptive in nature, in which the results of making cytological preparations in the form of capillary smears of human blood are stained with butterfly pea flower infusion at a concentration of 10%. The stained spots were then observed using a light microscope at 400x magnification. The colouring results of butterfly pea flowers were compared with a standard dye, Giemsa dye. The results showed that a 10% concentration of butterfly pea flowers stained erythrocytes but did not stain leukocytes well, while Giemsa's staining significantly stained well. The results also showed that butterfly pea flower infusion could only differentiate stained erythrocytes and unstained leukocytes but could not differentiate the types of leukocytes. Further research can be carried out by extracting anthocyanins from butterfly pea flowers and fractionating them to isolate single anthocyanin compounds that can potentially be dyed.

References

Angriani, L. (2019). The Potential of Extract Butterfly Pea Flower (Clitoria ternatea L.) as a Local Natural Dye for Various Food Industry. Canrea Journal: Food Technology, Nutritions, and Culinary Journal, 2(1), 32–37. https://doi.org/10.20956/canrea.v2i1.120

Charurungsipong, P., Tangduangdee, C., Amornraksa, S., Asavasanti, S., & Lin, J. (2020). Improvement of Anthocyanin Stability in Butterfly Pea Flower Extract by Co-pigmentation with Catechin. E3S Web of Conferences, 141, 3–6. https://doi.org/10.1051/e3sconf/202014103008

Islawati, Asriyani Ridwan, & Rahmat Aryandi. (2021). Ekstrak Betasianin dari Umbi Bit (Beta vulgaris) sebagai Pewarna Alami pada Sediaan Apusan Darah Tepi. Jurnal Kesehatan Panrita Husada, 6(2), 152–160. https://doi.org/10.37362/jkph.v6i2.644

Khasanah, N. A. H., Husen, F., & Yuniati, N. I. (2023). Pewarnaan Sediaan Apusan Darah Tepi (SADT) Menggunakan Infusa Bunga Telang (Clitorea ternatea). Jurnal Kesehatan Dan Science, 19(1), 67–78.

Marpaung, A. M. (2020). Tinjauan manfaat bunga telang (clitoria ternatea l.) bagi kesehatan manusia. Journal of Functional Food and Nutraceutical, 1(2), 63–85. https://doi.org/10.33555/jffn.v1i2.30

Nirmala Sari, A., Tazkiya, A., & Mafira, Y. (2022). Ekstrak Air Bunga Kencana Ungu (Ruellia simplex) Sebagai Pewarnaan Alternatif Preparat Sediaan Apusan Darah Tepi (Sadt). Prosiding Seminar Nasional Biotik, 9(2), 195. https://doi.org/10.22373/pbio.v9i2.11662

Nur Oktavia, S., Wahyuningsih, E., & Deti Andasari, S. (2020). Skrining Fitokimia Dari Infusa Dan Ekstrak Etanol 70% Daun Cincau Hijau(Cyclea barbata Miers). Jurnal Ilmu Farmasi, 11(1), 2685–1229.

Ratnaningtyas, N. I., Hernayanti, H., Ekowati, N., & Husen, F. (2022). Ethanol extract of the mushroom Coprinus comatus exhibits antidiabetic and antioxidant activities in streptozotocin-induced diabetic rats. Pharmaceutical Biology, 60(1), 1126–1136. https://doi.org/10.1080/13880209.2022.2074054

Rivenson, Y., Liu, T., Wei, Z., Zhang, Y., de Haan, K., & Ozcan, A. (2019). PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning. Light: Science and Applications, 8(1), 1–11. https://doi.org/10.1038/s41377-019-0129-y

Rofiqoh, & Taufikurohmah. (2013). Pengaruh Infiltrasi Nanogold Terhadap Peningkatan Kualitas Jaringan Dan Kuantitas Merkuri Pada Lambung Mencit (Mus Musculus) Setelah Terpapar Merkuri (Effect On Nanogold Infiltration Quantity And Quality Improvement Mercury In Mice Stomach (Mus Musculus). UNESA Journal of Chemistry, 2(3).

Sumartini, & Ikrawan, Y. (2020). Analisis Bunga Telang (Clitoria ternatea) Dengan Variasi Ph Metode Liquid Chromatograph-Tandem Mass Spectrometry (Lc-Ms/Ms) Sumartini Sumartini. Pasundan Food Technology Journal, 7(2), 70–77. https://doi.org/10.23969/pftj.v7i2.2983

Suryana, M. R. (2021). Ekstraksi Antosianin Pada Bunga Telang (Clitoria Ternatea L.): Sebuah Ulasan. Pasundan Food Technology Journal, 8(2), 45–50. https://doi.org/10.23969/pftj.v8i2.4049

Tirtasari, N. L., & Prasetya, T. (2020). Pengaruh Rasio Berat Bunga Telang (Clitoria ternatea. L) dan Volume Pelarut Asam Sitrat terhadap Pewarnaan Preparat Jaringan Tumbuhan. Indonesian Journal of Chemical Science, 9(3), 201–204.

Wantini, S., & Huda, M. (2021). Pengaruh Konsentrasi dan Waktu Pengecatan Giemsa Pada Pemeriksaan Mikroskopik Malaria The Effect Of Concentration And Time Of Giemsa Staining On Microscopic Examination Of Malaria. 10(1).

Yulianingsih Anwar, A., & Nurhamsiah. (2018). Penentuan Kriteria Penilaian Kesan Jumlah Leukosit Pada Pemeriksaan Apusan Darah Tepi. Jurnal Kesehatan Panrita Husada, 3(2), 27–34. https://doi.org/10.37362/jkph.v3i2.156